… (iii) heterologous competitors avoid the formation of … CRT-PCR is an extremely flexible and
sensitive assay that can quantify MDR1 based on competitive amplification of a heterologous …

… Heterologous DNA competitors were designed based on … fashion, we employed a quantitative
PCR approach. The … increased MDR1 transcription measured by quantitative RT-PCR (Fig…

… Absolute quantitation by RT-PCR, or “competitive RT-PCR,” is currently the most sensitive
and rigorous way to quantitate … requires the addition of known amounts of a synthetic transcript…

… polymerase chain reaction (PCR) is a more sensitive method … quantification of MDR1 gene
expression was done on … the MDR-1 data to the number of GAPDH transcripts measured in …

… reverse transcription (RT)-PCR with four internal competitors … and reach an absolute
quantitation of DNA and to compensate … In this study, we have described a sensitive RT-PCR …

… Absolute quantitation of MDR1 transcripts using heterologous DNA standards-validation of
the competitive RT-PCR (CRT-PCR) approach. BioTechniques 26, 1114–1124 25 Adachi, M.…

… by immunoblot and for PlGF by comparative reverse transcription-PCR. During weeks 2–5 …
PlGF mRNA was faintly detectable by comparative RT-PCR (see “Materials and Methods”); …

… reaction (RT-PCR) to evaluating the MDR1 gene expression … results of RT-PCR with the
findings of other methods used for … These results recommend these method for clinical practice …

… MDR– 1 gene mRNA is reported to be more sensitive than measurement of p-gp protein. The
aim of this study is to evaluate whether MDR–1 … mRNA expression levels of MDR-1 gene in …